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anti mouse cd38 pe vio770  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec anti mouse cd38 pe vio770
    Anti Mouse Cd38 Pe Vio770, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd38/CD38+Antibody%2C+anti-mouse/pmc13337242-96-3-7
    Average 93 stars, based on 10 article reviews
    anti mouse cd38 pe vio770 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    other:

    Article Title: Exercise-mobilized lymphocytes enhance antibody-based immunotherapy in multiple myeloma through CD16 + NK cell-mediated cytotoxicity.
    Article Snippet: Surface and intracellular staining on lymphocytes and tumors Frozen PBMCs were thawed and pretreated for 24 h with daratumumab or magrolimab (10 μg/mL; Selleckchem, USA), lenalidomide (2 μM; Selleckchem, USA), and dexamethasone (50

    Flow Cytometry:

    Article Title: Temporal Genomic Dynamics Shape Clinical Trajectory in Multiple Myeloma
    Article Snippet: Tumor cells from diagnostic samples were isolated from diagnostic samples by CD138 magnetic beads using either a QuadroMACS separator and LS column-based method (CD138 MicroBeads, human, Mylteni Biotec, USA) or a RobosepTM approach (EasySepTM Human CD138 Positive Selection Kit II, Stemcell, Canada) according to manufacturer’s instruction. .. Tumor cells from follow-up samples were sorted by flow cytometry based on the expression of CD38 (PE anti-human CD38 Antibody, Miltenyi, USA, 1:100), CD138 (APC anti-human CD138 Antibody, Biolegend, USA, 1:100), or CD45 (FITC Antibody, Miltenyi, USA, 1:200), with an additional Live/Dead exclusion (DAPI, Invitrogen, USA) using a BD FACS-Aria III cell sorter. .. FCR blocking was ensured using FcR Blocking (Human, Miltenyi).

    Expressing:

    Article Title: Temporal Genomic Dynamics Shape Clinical Trajectory in Multiple Myeloma
    Article Snippet: Tumor cells from diagnostic samples were isolated from diagnostic samples by CD138 magnetic beads using either a QuadroMACS separator and LS column-based method (CD138 MicroBeads, human, Mylteni Biotec, USA) or a RobosepTM approach (EasySepTM Human CD138 Positive Selection Kit II, Stemcell, Canada) according to manufacturer’s instruction. .. Tumor cells from follow-up samples were sorted by flow cytometry based on the expression of CD38 (PE anti-human CD38 Antibody, Miltenyi, USA, 1:100), CD138 (APC anti-human CD138 Antibody, Biolegend, USA, 1:100), or CD45 (FITC Antibody, Miltenyi, USA, 1:200), with an additional Live/Dead exclusion (DAPI, Invitrogen, USA) using a BD FACS-Aria III cell sorter. .. FCR blocking was ensured using FcR Blocking (Human, Miltenyi).

    FACS:

    Article Title: Temporal Genomic Dynamics Shape Clinical Trajectory in Multiple Myeloma
    Article Snippet: Tumor cells from diagnostic samples were isolated from diagnostic samples by CD138 magnetic beads using either a QuadroMACS separator and LS column-based method (CD138 MicroBeads, human, Mylteni Biotec, USA) or a RobosepTM approach (EasySepTM Human CD138 Positive Selection Kit II, Stemcell, Canada) according to manufacturer’s instruction. .. Tumor cells from follow-up samples were sorted by flow cytometry based on the expression of CD38 (PE anti-human CD38 Antibody, Miltenyi, USA, 1:100), CD138 (APC anti-human CD138 Antibody, Biolegend, USA, 1:100), or CD45 (FITC Antibody, Miltenyi, USA, 1:200), with an additional Live/Dead exclusion (DAPI, Invitrogen, USA) using a BD FACS-Aria III cell sorter. .. FCR blocking was ensured using FcR Blocking (Human, Miltenyi).

    Staining:

    Article Title: Early-stage B cells predict relapse after rituximab treatment in patients with Membranous Nephropathy
    Article Snippet: .. To characterize B-cell subsets, peripheral blood mononuclear cells were stained with fluorochrome-conjugated monoclonal antibodies (BD Biosciences) directed against the following antigens: CD45 (V500-C), CD3 (APC), CD19 (APC-H7), CD27 (BV421), IgD (PE), CD38 (BV711), CD4 (BV605), and CD8 (PE), as well as 7-AAD (Miltenyi Biotech). .. The stained cells were then analyzed using multicolor flow cytometry (BD FACS Lyric).

    Article Title: Early-Stage B-cells Predict Relapse After Rituximab Treatment in Patients With Membranous Nephropathy
    Article Snippet: .. To characterize B-cell subsets, peripheral blood mononuclear cells were stained with fluorochrome-conjugated monoclonal antibodies (BD Biosciences) directed against the following antigens: CD45 (V500-C), CD3 (APC), CD19 (APC-H7), CD27 (BV421), IgD (PE), CD38 (BV711), CD4 (BV605), and CD8 (PE), as well as 7-AAD (Miltenyi Biotech). .. The stained cells were then analyzed using multicolor flow cytometry (BD FACS Lyric).

    Bioprocessing:

    Article Title: Early-stage B cells predict relapse after rituximab treatment in patients with Membranous Nephropathy
    Article Snippet: .. To characterize B-cell subsets, peripheral blood mononuclear cells were stained with fluorochrome-conjugated monoclonal antibodies (BD Biosciences) directed against the following antigens: CD45 (V500-C), CD3 (APC), CD19 (APC-H7), CD27 (BV421), IgD (PE), CD38 (BV711), CD4 (BV605), and CD8 (PE), as well as 7-AAD (Miltenyi Biotech). .. The stained cells were then analyzed using multicolor flow cytometry (BD FACS Lyric).

    Article Title: Early-Stage B-cells Predict Relapse After Rituximab Treatment in Patients With Membranous Nephropathy
    Article Snippet: .. To characterize B-cell subsets, peripheral blood mononuclear cells were stained with fluorochrome-conjugated monoclonal antibodies (BD Biosciences) directed against the following antigens: CD45 (V500-C), CD3 (APC), CD19 (APC-H7), CD27 (BV421), IgD (PE), CD38 (BV711), CD4 (BV605), and CD8 (PE), as well as 7-AAD (Miltenyi Biotech). .. The stained cells were then analyzed using multicolor flow cytometry (BD FACS Lyric).



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    Image Search Results


    Enhanced CD38 ecto‐NADase activity in BMMs during skeletal aging impairs NMN availability to BMSCs. (a) qPCR analysis of Cd38 , Cdkn2a , Cdkn1a , Nos2 , Tnfα , IL1b , IL6 , Ym1 , Fizz1 , and Arg1 mRNA levels in bone marrow from 3‐ or 18‐month‐old WT and 18‐month‐old Sting ΔMφ mice ( n = 6 biological replicates). (b—d) Representative micro‐CT images (b) and quantitative analysis of BV/TV, Tb. N, Tb.Th, and Tb.Sp (c,d) from AAV9‐BMM‐shNC‐treated or AAV9‐BMM‐sh Cd38 ‐treated male mice ( n = 6 biological replicates). (e) WST‐8 analysis of NAD + levels in 3, 10, 18, and 24 M‐BMSCs ( n = 6 biological replicates). (f) WST‐8 analysis of NAD + levels in BMSCs under co‐culture conditions ( n = 6 biological replicates). (g) WST‐8 analysis of NAD + levels in BMSCs from the bone marrow of mice treated with PBS or 78c from 16‐ to 18‐month‐old ( n = 6 biological replicates). (h) WST‐8 analysis of NAD + levels in BMSCs from the bone marrow of mice injected with AAV9 for BMM‐specific overexpression of WT or Δ49 mouse Cd38 ( n = 6 biological replicates). Data are shown as mean ± SD.

    Journal: Advanced Science

    Article Title: Bone Marrow Macrophage STING Signaling is a Direct Nexus Linking Marrow Adipose Expansion to NAD + Depletion and Osteogenic Decline in Skeletal Aging

    doi: 10.1002/advs.77571

    Figure Lengend Snippet: Enhanced CD38 ecto‐NADase activity in BMMs during skeletal aging impairs NMN availability to BMSCs. (a) qPCR analysis of Cd38 , Cdkn2a , Cdkn1a , Nos2 , Tnfα , IL1b , IL6 , Ym1 , Fizz1 , and Arg1 mRNA levels in bone marrow from 3‐ or 18‐month‐old WT and 18‐month‐old Sting ΔMφ mice ( n = 6 biological replicates). (b—d) Representative micro‐CT images (b) and quantitative analysis of BV/TV, Tb. N, Tb.Th, and Tb.Sp (c,d) from AAV9‐BMM‐shNC‐treated or AAV9‐BMM‐sh Cd38 ‐treated male mice ( n = 6 biological replicates). (e) WST‐8 analysis of NAD + levels in 3, 10, 18, and 24 M‐BMSCs ( n = 6 biological replicates). (f) WST‐8 analysis of NAD + levels in BMSCs under co‐culture conditions ( n = 6 biological replicates). (g) WST‐8 analysis of NAD + levels in BMSCs from the bone marrow of mice treated with PBS or 78c from 16‐ to 18‐month‐old ( n = 6 biological replicates). (h) WST‐8 analysis of NAD + levels in BMSCs from the bone marrow of mice injected with AAV9 for BMM‐specific overexpression of WT or Δ49 mouse Cd38 ( n = 6 biological replicates). Data are shown as mean ± SD.

    Article Snippet: In parallel, 16‐month‐old male C57BL/6J mice received 78c (MedChemExpress, HY‐123999; 10 mg/kg) or PBS intraperitoneally 5 days per week for 8 weeks.

    Techniques: Activity Assay, Analysis, Micro-CT, Co-culture Assay, Injection, Over Expression